秀丽隐杆线虫表达序列标签可识别基因家族和潜在的疾病基因同源物。
Caenorhabditis elegans expressed sequence tags identify gene families and potential disease gene homologues.
作者信息
McCombie W R, Adams M D, Kelley J M, FitzGerald M G, Utterback T R, Khan M, Dubnick M, Kerlavage A R, Venter J C, Fields C
机构信息
Receptor Biochemistry and Molecular Biology Section, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, Maryland 20892.
出版信息
Nat Genet. 1992 May;1(2):124-31. doi: 10.1038/ng0592-124.
A database containing mapped partial cDNA sequences from Caenorhabditis elegans will provide a ready starting point for identifying nematode homologues of important human genes and determining their functions in C. elegans. A total of 720 expressed sequence tags (ESTs) have been generated from 585 clones randomly selected from a mixed-stage C. elegans cDNA library. Comparison of these ESTs with sequence databases identified 422 new C. elegans genes, of which 317 are not similar to any sequences in the database. Twenty-six new genes have been mapped by YAC clone hybridization. Members of several gene families, including cuticle collagens, GTP-binding proteins, and RNA helicases were discovered. Many of the new genes are similar to known or potential human disease genes, including CFTR and the LDL receptor.
一个包含秀丽隐杆线虫部分cDNA序列定位信息的数据库，将为鉴定重要人类基因的线虫同源物以及确定它们在秀丽隐杆线虫中的功能提供一个现成的起点。从一个混合发育阶段的秀丽隐杆线虫cDNA文库中随机挑选出585个克隆，共产生了720个表达序列标签（EST）。将这些EST与序列数据库进行比较，鉴定出422个新的秀丽隐杆线虫基因，其中317个与数据库中的任何序列都不相似。通过YAC克隆杂交对26个新基因进行了定位。发现了几个基因家族的成员，包括表皮胶原蛋白、GTP结合蛋白和RNA解旋酶。许多新基因与已知的或潜在的人类疾病基因相似，包括囊性纤维化跨膜传导调节因子（CFTR）和低密度脂蛋白受体。
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