[杀菌剂1,3 - 二硫杂环戊烷 - 2 - 亚基肼基苯甲酸的体外代谢研究]
[Studies on metabolism of fungicide benzoic acid, 1,3-dithiolan-2-ylidenehydrazide in vitro].
作者信息
Gao N
机构信息
Department of Environmental Health, Third Military Medical College, Chongqing.
出版信息
Zhonghua Yu Fang Yi Xue Za Zhi. 1992 Jul;26(4):223-6.
The metabolism of fungicide benzoic acid, 1,3-dithiolan-2-ylidenehydrazied (Yekuling) was studied quantitatively in rat liver microsomes and liver soluble fractions pretreated with phenobarbital (PB) and 3-methylcholanthrene (3-MC) by high pressure liquid chromatography. The experimental results indicated that the major metabolic pathway of Yekuling in vitro was hydrolysis. PB can enhance amidase activity to increase formation of benzoic acid and 1,3-dithiolan-2-ylidenehydrazine. 3-MC treatment elevated rat liver microsomal cytochrome P-448, enhancing S-oxidation of Yekuling. On the other hand, S-oxidation of Yekuling by rat liver microsomal MFO was NADPH-dependent.
采用高压液相色谱法，对经苯巴比妥（PB）和3-甲基胆蒽（3-MC）预处理的大鼠肝脏微粒体和肝脏可溶性组分中杀菌剂苯甲酸1,3-二硫杂环戊烯-2-亚基肼（叶枯灵）的代谢进行了定量研究。实验结果表明，叶枯灵在体外的主要代谢途径是水解。PB可增强酰胺酶活性，增加苯甲酸和1,3-二硫杂环戊烯-2-亚基肼的生成。3-MC处理可提高大鼠肝脏微粒体细胞色素P-448水平，增强叶枯灵的S-氧化作用。另一方面，大鼠肝脏微粒体混合功能氧化酶对叶枯灵进行S-氧化作用依赖于NADPH。
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