糖皮质激素、雄激素和视黄酸对仓鼠平滑肌肿瘤细胞中谷胱甘肽S-转移酶基因表达的调控

Glucocorticoid, androgen, and retinoic acid regulation of glutathione S-transferase gene expression in hamster smooth muscle tumor cells.

作者信息

Schwartz D A, Norris J S

机构信息

Department of Medicine, Medical University of South Carolina, Charleston 29425.

出版信息

Biochem Biophys Res Commun. 1992 Apr 30;184(2):1108-13. doi: 10.1016/0006-291x(92)90706-q.

DOI:10.1016/0006-291x(92)90706-q

PMID:1315523

Abstract

A mu class glutathione S-transferase gene (hGSTYBX) is expressed in the DDT1MF-2 hamster smooth muscle tumor cell line. This gene is glucocorticoid responsive, and near maximal induction was found to occur within 24 h. The induced mRNA was very stable with a half-life of more than 48 h. Serum had no effect on either constitutive or glucocorticoid induced hGSTYBX expression. Although dibutyryl cAMP, phenobarbital, and 12-O-tetradecanoylphorbol-13-acetate did not alter hGSTYBX expression, testosterone and retinoic acid were each able to increase hGSTYBX expression in a concentration dependent manner. These results demonstrate a unique pattern of responsiveness of the hamster gene compared to the glutathione S-transferase genes of other species.

摘要

一个μ类谷胱甘肽S-转移酶基因（hGSTYBX）在DDT1MF-2仓鼠平滑肌肿瘤细胞系中表达。该基因对糖皮质激素有反应，并且发现接近最大诱导在24小时内发生。诱导的mRNA非常稳定，半衰期超过48小时。血清对组成型或糖皮质激素诱导的hGSTYBX表达均无影响。虽然二丁酰环磷腺苷、苯巴比妥和12-O-十四酰佛波醇-13-乙酸酯不会改变hGSTYBX表达，但睾酮和视黄酸各自能够以浓度依赖的方式增加hGSTYBX表达。与其他物种的谷胱甘肽S-转移酶基因相比，这些结果证明了仓鼠基因独特的反应模式。