钙离子依赖的胞质蛋白向人中性粒细胞分离的颗粒亚群和质膜的转位。

Ca(2+)-dependent translocation of cytosolic proteins to isolated granule subpopulations and plasma membrane from human neutrophils.

作者信息

Borregaard N, Kjeldsen L, Lollike K, Sengeløv H

机构信息

Department of Hematology, University Hospital, Copenhagen, Denmark.

出版信息

FEBS Lett. 1992 Jun 15;304(2-3):195-7. doi: 10.1016/0014-5793(92)80617-p.

DOI:10.1016/0014-5793(92)80617-p

PMID:1319926

Abstract

In order to identify cytosolic proteins involved in control of granule exocytosis in human neutrophils, subcellular fractions enriched in each of the 3 major granule subsets were incubated with cytosol from neutrophils in the presence or absence of Ca2+. After washing, proteins were eluted from the organelles by EGTA. Annexins I, II, IV and VI were found to bind to all organelles studied. In addition, a 28-kDa protein was found to bind exclusively to plasma membranes and secretory vesicles, the most readily exocytosed organelle of neutrophils. Ca(2+)-dependent association of cytosolic proteins to different granule subsets may control differential exocytosis of granules.

摘要

为了鉴定参与人类中性粒细胞颗粒胞吐作用调控的胞质蛋白，将富含3种主要颗粒亚群中每一种的亚细胞组分与中性粒细胞的胞质溶胶在有或无Ca2+存在的情况下进行孵育。洗涤后，通过乙二醇双四乙酸（EGTA）从细胞器中洗脱蛋白质。发现膜联蛋白I、II、IV和VI可与所有研究的细胞器结合。此外，还发现一种28 kDa的蛋白质仅与质膜和分泌小泡结合，分泌小泡是中性粒细胞最容易发生胞吐作用的细胞器。胞质蛋白与不同颗粒亚群的Ca(2+)依赖性结合可能控制颗粒的差异性胞吐作用。