锌对HepG2细胞中刺激促红细胞生成素合成的抑制作用。

Inhibitory effect of zinc on stimulated erythropoietin synthesis in HepG2 cells.

作者信息

Dittmer J, Bauer C

机构信息

Physiologisches Institut, Universität Zürich-Irchel, Switzerland.

出版信息

Biochem J. 1992 Jul 1;285 ( Pt 1)(Pt 1):113-6. doi: 10.1042/bj2850113.

DOI:10.1042/bj2850113

PMID:1322122

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1132752/

Abstract

The effect of zinc on erythropoietin (EPO) synthesis in HepG2 cells was investigated. The increase in EPO synthesis induced by Co2+ (50 microM), Ni2+ (300 microM) or oxygen (1% O2) was inhibited by the presence of ZnCl2 (50-150 microM) in the tissue-culture medium, whereas basal EPO synthesis was unaffected. The effect was reflected by corresponding changes in the EPO mRNA level. These effects of zinc on EPO synthesis could not be mimicked by CdCl2 (less than or equal to 2 microM). Addition of FeCl3 to the medium appeared to decrease the inhibitory effect of zinc on hypoxia-induced EPO synthesis, implying that zinc may interfere with an iron-dependent step in EPO regulation.

摘要

研究了锌对HepG2细胞中促红细胞生成素（EPO）合成的影响。组织培养基中存在ZnCl2（50 - 150 microM）时，由Co2 +（50 microM）、Ni2 +（300 microM）或氧气（1% O2）诱导的EPO合成增加受到抑制，而基础EPO合成不受影响。这种效应反映在EPO mRNA水平的相应变化上。CdCl2（小于或等于2 microM）无法模拟锌对EPO合成的这些影响。向培养基中添加FeCl3似乎降低了锌对缺氧诱导的EPO合成的抑制作用，这意味着锌可能干扰EPO调节中依赖铁的步骤。