细胞色素b558是吞噬细胞NADPH氧化酶的一个组成部分，是一种黄素蛋白。
Cytochrome b558, a component of the phagocyte NADPH oxidase, is a flavoprotein.
作者信息
Sumimoto H, Sakamoto N, Nozaki M, Sakaki Y, Takeshige K, Minakami S
机构信息
Department of Biochemistry, Kyushu University School of Medicine, Fukuoka, Japan.
出版信息
Biochem Biophys Res Commun. 1992 Aug 14;186(3):1368-75. doi: 10.1016/s0006-291x(05)81557-8.
Cytochrome b558 is the only membrane component of the phagocyte O2(-)-producing NADPH oxidase. The O2- production by the oxidase reconstituted in vitro with the crude membrane fraction is enhanced several-fold by addition of FAD, whereas that with the partially purified cytochrome is completely dependent on exogenous FAD, suggesting that FAD acts through the membrane component, cytochrome b558. The alignments of the amino acid sequence of the large subunit of the cytochrome (gp91-phox) with those of previously characterized flavoproteins reveal that the middle and C-terminal portions of gp91-phox are likely to be FAD- and NADPH-binding domains, respectively. Cytochrome b558, thus, appears to be a flavoprotein with an NADPH-binding site, of the NADPH oxidase.
细胞色素b558是吞噬细胞产生超氧阴离子（O2-）的NADPH氧化酶的唯一膜成分。用粗膜组分在体外重建的氧化酶产生O2-的能力，通过添加FAD可增强几倍，而用部分纯化的细胞色素时，其产生O2-的能力则完全依赖于外源FAD，这表明FAD通过膜成分细胞色素b558起作用。细胞色素（gp91-phox）大亚基的氨基酸序列与先前鉴定的黄素蛋白的氨基酸序列比对显示，gp91-phox的中部和C端部分可能分别是FAD和NADPH结合结构域。因此，细胞色素b558似乎是一种具有NADPH结合位点的黄素蛋白，属于NADPH氧化酶。
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