Brown J E, Faddis M, Combs A
Department of Ophthalmology and Visual Sciences, Washington University, St Louis, MO 63110.
Exp Eye Res. 1992 Mar;54(3):403-10. doi: 10.1016/0014-4835(92)90052-t.
Both slices of squid (Loligo) retinas and Limulus ventral photoreceptors, with or without prior stimulation by flashes of bright light, were rapidly frozen and analysed for cyclic-GMP content by radioimmunoassay. Measurement of the freezing process indicated that freezing occurred at about 100 msec after the light flash. No significant changes in cyclic-GMP content were induced by light in either tissue. These data do not confirm previous reports that cyclic-GMP content of invertebrate photoreceptors is markedly increased by illumination.
将鱿鱼(枪乌贼属)视网膜切片和鲎腹侧光感受器,无论是否事先用强光闪光刺激,迅速冷冻,并通过放射免疫测定法分析其环磷酸鸟苷含量。对冷冻过程的测量表明,冷冻在闪光后约100毫秒发生。光照在这两种组织中均未引起环磷酸鸟苷含量的显著变化。这些数据不支持先前关于无脊椎动物光感受器的环磷酸鸟苷含量因光照而显著增加的报道。