Sugawara I, Hirata A, Ueda K, Itoyama S
Department of Pathology, Saitama Medical Center, Saitama Medical School.
Jpn J Cancer Res. 1992 Aug;83(8):795-7. doi: 10.1111/j.1349-7006.1992.tb01982.x.
A murine monoclonal antibody (MDR3M) (isotype: IgM) reactive with mdr3 gene product was generated by immunizing mice with mdr3-specific peptide (H2N-12WRPTSAEGDFELGISSKQKRKKTKTVKMI41G-COOH) and hybridizing the primed mouse splenic B cells with X63-Ag8,6.5.3 mouse plasmacytoma cells. MDR3M did not cross-react with mdr1 gene product. This monoclonal antibody may be useful for analyzing the role of mdr3 gene product in cells and tissues.
通过用mdr3特异性肽(H2N-12WRPTSAEGDFELGISSKQKRKKTKTVKMI41G-COOH)免疫小鼠,并将致敏的小鼠脾B细胞与X63-Ag8,6.5.3小鼠骨髓瘤细胞杂交,产生了一种与mdr3基因产物反应的鼠单克隆抗体(MDR3M)(同种型:IgM)。MDR3M与mdr1基因产物无交叉反应。这种单克隆抗体可能有助于分析mdr3基因产物在细胞和组织中的作用。