禽成髓细胞瘤相关病毒逆转录病毒蛋白酶的结构研究。

Structural studies of the retroviral proteinase from avian myeloblastosis associated virus.

作者信息

Ohlendorf D H, Foundling S I, Wendoloski J J, Sedlacek J, Strop P, Salemme F R

机构信息

Du Pont Merck Pharmaceutical Company, Wilmington, Delaware 19880-0228.

出版信息

Proteins. 1992 Nov;14(3):382-91. doi: 10.1002/prot.340140307.

DOI:10.1002/prot.340140307

PMID:1332025

Abstract

The structure of the retroviral proteinase from avian myeloblastosis associated virus (MAV) has been determined and refined at 2.2 A resolution. This structure is compared with those of homologous proteinases from Rous sarcoma virus (RSV) and human immunodeficiency type 1 virus (HIV). Through comparison with the structure of a proteinase-inhibitor complex from HIV, a model of a complex between MAV proteinase and a peptide substrate has been generated. Examination of this model suggests structural basis for the diverse specifications of viral proteinases.

摘要

禽成髓细胞瘤相关病毒（MAV）逆转录病毒蛋白酶的结构已被测定，并在2.2埃分辨率下进行了优化。该结构与劳斯肉瘤病毒（RSV）和人类免疫缺陷病毒1型（HIV）的同源蛋白酶结构进行了比较。通过与HIV蛋白酶-抑制剂复合物的结构比较，构建了MAV蛋白酶与肽底物复合物的模型。对该模型的研究揭示了病毒蛋白酶不同特异性的结构基础。