Cloning of a B2 bradykinin receptor: examination of the bradykinin binding site by site directed mutagenesis.

A cDNA encoding a bradykinin receptor has been isolated. In oocytes expressing the receptor, bradykinin-induced chloride current is blocked by [Thi5,8 dPhe7]BK and is unaffected by des-Arg9-BK suggesting that the cDNA encodes a classical B2 type receptor. The predicted protein sequence is homologous to other G protein-coupled receptors. Preliminary models of the receptor and BK have been built. Data from mutagenesis experiments designed to test the models is reported.