Gout E, Bligny R, Genix P, Tissut M, Douce R
Laboratoire de Résonance Magnétique en Biologie et Médecine, Centre d'Etudes Nucléaires, Grenoble, France.
Biochimie. 1992 Sep-Oct;74(9-10):875-82. doi: 10.1016/0300-9084(92)90071-l.
The effect of glyphosate (N-phosphonomethyl glycine; the active ingredient of Roundup herbicide) on plant cells metabolism was analysed by 31P and 13C NMR using suspension-cultured sycamore (Acer pseudoplatanus L) cells. Cells were compressed in the NMR tube and perfused with an original arrangement enabling a tight control of the circulating nutrient medium. Addition of 1 mM glyphosate to the nutrient medium triggered the accumulation of shikimate (20-30 mumol g-1 cell wet weight within 50 h) and shikimate 3-phosphate (1-1.5 mumol g-1 cell wet weight within 50 h). From in vivo spectra it was demonstrated that these two compounds were accumulated in the cytoplasm where their concentrations reached potentially lethal levels. On the other hand, glyphosate present in the cytoplasmic compartment was extensively metabolized to yield aminomethylphosphonic acid which also accumulated in the cytoplasm. Finally, the results presented in this paper indicate that although the cell growth was stopped by glyphosate the cell respiration rates and the level of energy metabolism intermediates remained unchanged.
利用31P和13C核磁共振技术，以悬浮培养的梧桐（Acer pseudoplatanus L）细胞为材料，分析了草甘膦（N-膦酰基甲基甘氨酸；农达除草剂的活性成分）对植物细胞代谢的影响。将细胞装入核磁共振管中，并通过一种能严格控制循环营养培养基的原始装置进行灌注。向营养培养基中添加1 mM草甘膦会引发莽草酸（50小时内积累量达20 - 30 μmol g-1细胞湿重）和3-磷酸莽草酸（50小时内积累量达1 - 1.5 μmol g-1细胞湿重）的积累。从体内光谱可知，这两种化合物积累于细胞质中，其浓度达到了可能致死的水平。另一方面，细胞质区室中的草甘膦被大量代谢生成氨甲基膦酸，该物质也在细胞质中积累。最后，本文给出的结果表明，尽管草甘膦使细胞生长停止，但细胞呼吸速率和能量代谢中间产物水平并未改变。
