Peränen J
Institute of Biotechnology, University of Helsinki, Finland.
Biotechniques. 1992 Oct;13(4):546-9.
A simple and rapid method to affinity-purify and biotinylate antibodies was developed. The method utilizes separation of antigens by sodium dodecyl sulfate polyacrylamide gel electrophoresis, followed by transfer to nitrocellulose and binding of the antibodies to the specific antigen. The antibodies are biotinylated, while still bound to the antigen, thus avoiding the conjugation of the active antigen-binding sites of the antibodies. These antibodies have been successfully used in double-label immunofluorescence studies, but they should be likewise applicable in other immunological protocols.
开发了一种简单快速的亲和纯化抗体并进行生物素化的方法。该方法利用十二烷基硫酸钠聚丙烯酰胺凝胶电泳分离抗原，随后转移至硝酸纤维素膜上，使抗体与特定抗原结合。抗体在仍与抗原结合时进行生物素化，从而避免了抗体活性抗原结合位点的缀合。这些抗体已成功用于双标记免疫荧光研究，但它们同样应适用于其他免疫学实验方案。
