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借助银浸染法对鸡胚结缔组织中纤维形成的观察。

Observations on fibrillogenesis in the connective tissue of the chick embryo with the aid of silver impregnation.

作者信息

KUBOTA L, WASSERMANN F

出版信息

J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):67-70. doi: 10.1083/jcb.2.4.67.

Abstract

A technique is described which combines silver impregnation and ultrathin sectioning for the electron microscopic demonstration of fibrils in the connective tissue of the chick embryo. The electron micrographs presented in this paper provide evidence for the specificity and completeness of the silver-impregnation technique. It has been shown that, in this particular tissue after fixation in neutral formalin and at the stage of development represented by our material, the argyrophil fibers are embedded in a material which is continuous with the body of the fibroblasts.

摘要

本文描述了一种将银浸染和超薄切片相结合的技术,用于在电子显微镜下显示鸡胚结缔组织中的原纤维。本文呈现的电子显微照片为银浸染技术的特异性和完整性提供了证据。结果表明,在以我们的材料所代表的发育阶段,用中性福尔马林固定后的这种特定组织中,嗜银纤维嵌入在一种与成纤维细胞体连续的物质中。

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[Histolysis of the cornea. Descemet's membrane].
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1969;179(2):157-74. doi: 10.1007/BF00410382.
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A simple staining technique for electron microscopy with lead-uranyl acetate.
Experientia. 1967 Apr 15;23(4):316-7. doi: 10.1007/BF02135708.

本文引用的文献

1
The morphogenesis of avian tendon.
Proc R Soc Lond B Biol Sci. 1956 Mar 13;144(917):556-72. doi: 10.1098/rspb.1956.0011.
3
Collagen, reticulum and their argyrophilic properties.
Proc R Soc Lond B Biol Sci. 1954 Feb 18;142(906):113-25. doi: 10.1098/rspb.1954.0009.
5
Reticulin and collagen.
Nature. 1953 Feb 14;171(4346):302. doi: 10.1038/171302a0.

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