兔背根神经节中岩藻糖基化糖缀合物的发育变化。

Developmental changes of fucosylated glycoconjugates in rabbit dorsal root ganglia.

作者信息

Kusunoki S, Inoue K, Iwamori M, Nagai Y, Mannen T, Kanazawa I

机构信息

Department of Neurology and Biochemistry, Faculty of Medicine, University of Tokyo, Japan.

出版信息

Neurosci Res. 1992 Oct;15(1-2):74-80. doi: 10.1016/0168-0102(92)90019-9.

DOI:10.1016/0168-0102(92)90019-9

PMID:1336587

Abstract

Developmental changes of the fucosylated glycoconjugates in the dorsal root ganglia (DRG) of the rabbit were investigated histochemically using anti-fucosyl GM1 antibody and Ulex europaeus agglutinin 1 (UEA-1) lectin. Neither anti-fucosyl GM1 antibody nor UEA-1 lectin bound to the neural tubes or to the neural crest on embryonic day 14 (E14). Anti-fucosyl GM1 antibody binds diffusely to the DRG of E25. Large neurons unreactive with anti-fucosyl GM1 antibody appeared at 1 month and increased within 6 months after birth. Schwann cells immunoreactive with anti-fucosyl GM1 antibody came to be limited to the satellite cells surrounding the positive neurons. No staining with UEA-1 lectin was observed in the DRG of E25. Some small neurons became reactive with UEA-1 lectin within 1 month and remained to be so at 6 months after birth. Schwann cells including satellite cells were unreactive with this lectin. Since fucosyl GM1 was detected in the lipid fraction of DRGs from 1-month-old and 6-month-old rabbits, fucosyl GM1 itself should be the antigen molecule recognized by the anti-fucosyl GM1 antibody. Further study is necessary to elucidate the association between these developmental changes of the fucosylated glycoconjugates in DRG and their possible functional roles.

摘要

利用抗岩藻糖基化GM1抗体和欧洲荆豆凝集素1（UEA-1）凝集素，通过组织化学方法研究了家兔背根神经节（DRG）中岩藻糖基化糖缀合物的发育变化。在胚胎第14天（E14），抗岩藻糖基化GM1抗体和UEA-1凝集素均未与神经管或神经嵴结合。抗岩藻糖基化GM1抗体在E25时弥漫性地结合于DRG。出生后1个月时出现对抗岩藻糖基化GM1抗体无反应的大神经元，并在出生后6个月内数量增加。与抗岩藻糖基化GM1抗体免疫反应的雪旺细胞局限于阳性神经元周围的卫星细胞。在E25的DRG中未观察到UEA-1凝集素染色。出生后1个月内一些小神经元开始对UEA-1凝集素产生反应，并在出生后6个月时仍保持反应性。包括卫星细胞在内的雪旺细胞对该凝集素无反应。由于在1月龄和6月龄家兔的DRG脂质部分中检测到岩藻糖基化GM1，岩藻糖基化GM1本身应是抗岩藻糖基化GM1抗体识别的抗原分子。需要进一步研究以阐明DRG中岩藻糖基化糖缀合物的这些发育变化与其可能的功能作用之间的关联。