5-羟色胺2型受体的激活会抑制大鼠脊髓中[3H]去甲肾上腺素的诱发释放。

Activation of 5-HT2 receptors inhibits the evoked release of [3H]noradrenaline in the rat spinal cord.

Celuch S M, Ramirez A J, Enero M A

Instituto de Investigaciones Farmacológicas, CONICET, Buenos Aires, Argentina.

Gen Pharmacol. 1992 Nov;23(6):1063-5. doi: 10.1016/0306-3623(92)90287-t.

Abstract

The participation of 5-HT2 receptors in the modulation of the evoked release of [3H]noradrenaline from rat spinal cord slices has been examined. 2. In rat spinal cord slices preincubated with [3H]noradrenaline, the alpha 2-receptor agonist clonidine (10(-6) mol/l) decreased the release of tritium evoked by field stimulation (600 pulses at 5 Hz, 20 mA, 2 msec), while the alpha 2-antagonist yohimbine (10(-6) mol/l) increased it. 3. The 5-HT2/5-HT1c agonist DOI (3 x 10(-7) mol/l) decreased the evoked release of tritium, an effect which was prevented by ketanserin (10(-7) mol/l). 4. It is suggested that in addition to presynaptic alpha 2-adrenoceptors, there are 5-HT2 receptors which modulate the release of noradrenaline in the rat spinal cord.

摘要

已对5-羟色胺2（5-HT2）受体在调节大鼠脊髓切片中[3H]去甲肾上腺素的诱发释放过程中的作用进行了研究。2. 在预先用[3H]去甲肾上腺素孵育的大鼠脊髓切片中，α2-受体激动剂可乐定（10^(-6)摩尔/升）可降低由场刺激（5赫兹、20毫安、2毫秒，600个脉冲）诱发的氚释放，而α2-拮抗剂育亨宾（10^(-6)摩尔/升）则可增加该释放。3. 5-HT2/5-HT1c激动剂DOI（3×10^(-7)摩尔/升）可降低诱发的氚释放，酮色林（10^(-7)摩尔/升）可阻止此效应。4. 提示除了突触前α2-肾上腺素能受体外，大鼠脊髓中还存在调节去甲肾上腺素释放的5-HT2受体。