Suppr超能文献

功能性MSH受体在培养的人黑素细胞上的表达。

The expression of functional MSH receptors on cultured human melanocytes.

作者信息

Donatien P D, Hunt G, Pieron C, Lunec J, Taïeb A, Thody A J

机构信息

Department of Dermatology, University of Newcastle upon Tyne, UK.

出版信息

Arch Dermatol Res. 1992;284(7):424-6. doi: 10.1007/BF00372074.

Abstract

Although alpha-MSH increases skin darkening in humans, there are several reports that it fails to have melanogenic effects on human melanocytes in vitro. The purpose of this study was to see whether cultured human melanocytes express MSH receptors. Human melanocytes were grown in the absence of artificial mitogens such as 12-O-tetradecanoyl phorbol-13-acetate (TPA) and cholera toxin (CT) and incubated for 2 h at room temperature with increasing amounts of 125I-labelled Nle4DPhe7-alpha-MSH with and without excess cold peptide. Binding was saturable and specific: Scatchard analysis gave a Kd of 4.9 x 10(-11) M and approximately 700 binding sites/cell. Human keratinocytes and fibroblasts showed no specific binding. The addition of 1 mM dibutyryl cAMP to the culture medium caused a 62% increase in MSH binding to human melanocytes. A smaller increase (25%) was seen with 10(-9) M CT while 25 mM TPA caused a 24% decrease. These results show that human melanocytes in culture express MSH receptors and that this expression can be modulated by mitogens.

摘要

尽管α-促黑素细胞激素(α-MSH)可使人类皮肤变黑,但有几份报告称,它在体外对人类黑素细胞没有致黑素生成作用。本研究的目的是观察培养的人类黑素细胞是否表达促黑素细胞激素(MSH)受体。人类黑素细胞在不含人工促细胞分裂剂(如12-O-十四烷酰佛波醇-13-乙酸酯(TPA)和霍乱毒素(CT))的情况下生长,并在室温下与不同量的125I标记的Nle4DPhe7-α-MSH一起孵育2小时,同时加入或不加入过量的冷肽。结合是饱和且特异的:Scatchard分析得出解离常数(Kd)为4.9×10^(-11) M,每个细胞约有700个结合位点。人类角质形成细胞和成纤维细胞未显示出特异性结合。向培养基中添加1 mM二丁酰环磷腺苷(dibutyryl cAMP)可使MSH与人类黑素细胞的结合增加62%。10^(-9) M CT可使结合有较小增加(25%),而25 mM TPA则使结合减少24%。这些结果表明,培养的人类黑素细胞表达MSH受体,且这种表达可被促细胞分裂剂调节。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验