Winkler C, Hong Y, Wittbrodt J, Schartl M
Max-Planck-Institüt für Biochemie, Martinsried, Germany.
Mol Mar Biol Biotechnol. 1992 Aug-Oct;1(4-5):326-37.
Efficient expression systems are required for analysis of gene regulation and function in teleost fish. To develop such systems, a number of inducible or constitutive promoter and enhancer sequences of fish or higher vertebrate origin were tested for activity in a variety of fish cell lines and in embryos of the Japanese medaka fish (Oryzias latipes) and Xiphophorus. The activity of the different promoter-enhancer combinations were quantitated. Considerable differences were found for some constructs if tested in vitro or in vivo. Fro the data obtained, a set of expression vectors for basic research as well as for aquaculture purposes were established.
为了分析硬骨鱼的基因调控和功能，需要高效的表达系统。为开发这样的系统，测试了许多鱼类或高等脊椎动物来源的诱导型或组成型启动子和增强子序列在多种鱼类细胞系以及日本青鳉鱼（Oryzias latipes）和剑尾鱼胚胎中的活性。对不同启动子-增强子组合的活性进行了定量分析。如果在体外或体内进行测试，某些构建体存在显著差异。根据获得的数据，建立了一套用于基础研究以及水产养殖目的的表达载体。
