Bellinati-Pires R, Salgado M M, Joazeiro P P, Carneiro-Sampaio M M
Seção de Imunologia, Instituto Adolfo Lutz, São Paulo, Brasil.
Mem Inst Oswaldo Cruz. 1992 Oct-Dec;87(4):575-81. doi: 10.1590/s0074-02761992000400018.
The few studies already published about phagocyte functions in Chediak-Higashi syndrome (CHS) has stated that neutrophils present slow rate of bacterial killing but normally ingest microorganisms. In the present study, both phagocytosis and killing of Staphylococcus aureus were verified to be delayed in neutrophils from two patients with CHS when these functions were simultaneously evaluated by a fluorochrome phagocytosis assay. Electron microscopic examination showed morphologic differences among neutrophils from CHS patients and normal neutrophils regarding the cytoplasmic structures and the aspects of the phagolysosomes. It was noteworthy the presence of giant phagolysosomes enclosing bacteria in active proliferation commonly observed in CHS neutrophils after 45 min of phagocytosis, which corresponded with the impaired bactericidal activity of these leukocytes. The present results suggest that phagocytosis may also be defective in CHS, and point out to the sensitivity of the fluorochrome phagocytosis assay and its application in clinical laboratories.
已发表的关于切迪阿克-希加什综合征（CHS）中吞噬细胞功能的少数研究表明，中性粒细胞对细菌的杀伤速度较慢，但通常能摄取微生物。在本研究中，当通过荧光吞噬试验同时评估两名CHS患者中性粒细胞的吞噬和对金黄色葡萄球菌的杀伤功能时，发现这两项功能均延迟。电子显微镜检查显示，CHS患者的中性粒细胞与正常中性粒细胞在细胞质结构和吞噬溶酶体方面存在形态学差异。值得注意的是，在吞噬45分钟后，CHS中性粒细胞中常见有包含活跃增殖细菌的巨大吞噬溶酶体，这与这些白细胞杀菌活性受损相一致。目前的结果表明，CHS中的吞噬作用可能也存在缺陷，并指出了荧光吞噬试验的敏感性及其在临床实验室中的应用。
