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蝗虫精子成熟过程中细胞核的精细结构

The fine structure of nuclei during sperm maturation in the locust.

作者信息

GIBBONS I R, BRADFIELD J R

出版信息

J Biophys Biochem Cytol. 1957 Mar 25;3(2):133-40. doi: 10.1083/jcb.3.2.133.

Abstract
  1. In the heads of maturing sperm of a locust the chromatin becomes arranged in a highly regular manner so as to produce many parallel lines about 60 A thick in longitudinal section and contiguous polygons in transverse section. 2. This configuration appears after fixation in osmium tetroxide, formaldehyde, or acetic acid; intermediate stages in its development are illustrated. 3. These electron micrographs are interpreted to mean that, during sperm maturation, the chromatin becomes formed into sheets and then into tubes running parallel with the long axis. 4. In the mature sperm head we have been unable so far to detect this structure. This may be because the chromatin becomes so compact during the shrinkage of the nucleus which occurs during formation of the mature sperm.
摘要
  1. 在蝗虫成熟精子的头部,染色质以高度规则的方式排列,以至于在纵切面上产生许多约60埃厚的平行线,在横切面上产生相邻的多边形。2. 这种构型在经四氧化锇、甲醛或乙酸固定后出现;文中展示了其发育的中间阶段。3. 这些电子显微镜照片被解释为意味着,在精子成熟过程中,染色质先形成薄片,然后形成与长轴平行的管状物。4. 在成熟精子头部,我们至今未能检测到这种结构。这可能是因为在成熟精子形成过程中细胞核收缩时,染色质变得过于致密。

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本文引用的文献

1
The fixation of cell nuclei by osmium tetroxide.
Biochim Biophys Acta. 1956 Dec;22(3):506-13. doi: 10.1016/0006-3002(56)90061-0.
3
An electron microscope study of spermatid differentiation in the toad, Bufo arenarum Hensel.
J Biophys Biochem Cytol. 1956 May 25;2(3):223-40. doi: 10.1083/jcb.2.3.223.
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Chromosomal structures in crayfish spermatocytes.
J Biophys Biochem Cytol. 1956 Mar 25;2(2):215-8. doi: 10.1083/jcb.2.2.215.
7
Observations on the fine structure of the macronucleus of Tokophrya infusionum.
J Biophys Biochem Cytol. 1955 Sep 25;1(5):421-8. doi: 10.1083/jcb.1.5.421.

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