VON BERTALANFFY L, MASIN M, MASIN F, KAPLAN L
Calif Med. 1957 Oct;87(4):248-51.
Early detection of malignant lesions of the cervix, a major problem in gynecology, has been made possible in more cases by the development of exfoliative cytology. Mass-screening programs have been impeded, however, by the demands on time and skill of the examiner as posed by conventional techniques.A new method in exfoliative cytology, using fluorescence microscopy, essentially reduces the time of processing as well as of scanning of specimens. Suspicious cells show flaming orange-red fluorescence of the cytoplasm on a black background, impressively distinct from normal cells and giving a warning signal to the examiner. This color reaction is based upon cytochemical changes-namely, the abundance of ribonucleic acid in vividly growing and especially malignant cells.Besides gynecological material, the method is applicable to other forms of malignant disease.
子宫颈恶性病变的早期检测是妇科的一个主要问题，脱落细胞学的发展使更多病例能够实现早期检测。然而，传统技术对检查者的时间和技能要求阻碍了大规模筛查计划的开展。脱落细胞学中的一种新方法，即使用荧光显微镜，从根本上减少了标本处理和扫描的时间。可疑细胞在黑色背景下显示出细胞质的火焰状橙红色荧光，与正常细胞形成鲜明对比，给检查者发出警告信号。这种颜色反应基于细胞化学变化，即活跃生长尤其是恶性细胞中丰富的核糖核酸。除了妇科材料外，该方法还适用于其他形式的恶性疾病。
