小鼠体内游离D-丙氨酸、D-脯氨酸和D-丝氨酸的存在情况。
The presence of free D-alanine, D-proline and D-serine in mice.
作者信息
Nagata Y, Yamamoto K, Shimojo T, Konno R, Yasumura Y, Akino T
机构信息
Department of Biochemistry, Sapporo Medical College, Hokkaido, Japan.
出版信息
Biochim Biophys Acta. 1992 Jan 23;1115(3):208-11. doi: 10.1016/0304-4165(92)90055-y.
The presence of free D-alanine, D-proline and D-serine was demonstrated in mammalian tissues, using a mutant mouse strain lacking D-amino acid oxidase. In the experiment, free amino acids from the kidney and serum were derivatized with 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (FDAA) to diastereomers, separated by two-dimensional thin-layer chromatography (TLC), and analysed by reversed-phase high-performance liquid chromatography (HPLC) for the resolution of D- and L-isomers. D/L ratios of alanine, proline and serine were obtained based on the peak areas of HPLC.
利用缺乏D-氨基酸氧化酶的突变小鼠品系，在哺乳动物组织中证实了游离D-丙氨酸、D-脯氨酸和D-丝氨酸的存在。在实验中，肾脏和血清中的游离氨基酸用1-氟-2,4-二硝基苯基-5-L-丙氨酸酰胺（FDAA）衍生为非对映异构体，通过二维薄层色谱（TLC）分离，并通过反相高效液相色谱（HPLC）分析以分离D-异构体和L-异构体。基于HPLC的峰面积获得丙氨酸、脯氨酸和丝氨酸的D/L比值。
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