通过菌落杂交和斑点酶免疫测定法检测来自肠道外感染的大肠杆菌菌株中的S菌毛
Determination of S fimbriae among Escherichia coli strains from extraintestinal infections by colony hybridization and dot enzyme immunoassay.
作者信息
Schönian G, Sokolowska-Köhler W, Bollmann R, Schubert A, Gräser Y, Presber W
机构信息
Medizinische Fakultät (Charité), Humboldt-Universität zu Berlin.
出版信息
Zentralbl Bakteriol. 1992 Jan;276(2):273-9. doi: 10.1016/s0934-8840(11)80014-9.
449 E. coli strains obtained from septic infections (124 isolates), urinary tract infections (246) and water (79) were surveyed for S/F1C fimbrial DNA by DNA hybridization and for expression of S fimbriae by enzyme immunoassay. S/F1C fimbrial DNA was detected with greater frequency in septic (35%) and urinary (43%) isolates than in water isolates and was often associated with the O2, O4, O6, O18, O83, and O156 serogroups. Most O45 strains did not possess such sequences. Only 12% and 28%, respectively, of the septic and urinary strains possessing S/F1C fimbrial DNA expressed S fimbriae.
通过DNA杂交检测从败血症感染（124株分离株）、尿路感染（246株）和水（79株）中获得的449株大肠杆菌菌株的S/F1C菌毛DNA，并通过酶免疫测定法检测S菌毛的表达。与水中分离株相比，败血症（35%）和尿液（43%）分离株中检测到S/F1C菌毛DNA的频率更高，且常与O2、O4、O6、O18、O83和O156血清群相关。大多数O45菌株不具有此类序列。拥有S/F1C菌毛DNA的败血症和尿液菌株中，分别只有12%和28%表达S菌毛。
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