Dumler J S, Wisseman C L
Department of Pathology, University of Texas Medical Branch, Galveston 77550.
Acta Virol. 1992 Jan;36(1):45-51.
The local perivascular mononuclear cell inflammatory infiltrate (PVI) response to intradermal (ID) challenge with viable R. prowazekii was studied in a typhus-immune subject. An immunohistochemical method for specific mononuclear cell markers was used on skin punch biopsies taken 6, 24 and 48 hr after challenge. By 24 to 48 hr, the histologic findings were consistent with a delayed-type hypersensitivity reaction (DTHR). Both CD4+ and CD8+ T lymphocytes dominated the PVI at 48 h. CD8+ cells entered the PVI more rapidly than CD4+ cells. Local control of R. prowazekii challenge in an immune human subject was associated with recruitment into the PVI of T lymphocytes which are rich sources of gamma-interferon, and CD8+ T cells which are potentially cytotoxic for R. typhi-infected cells.
在一名斑疹伤寒免疫受试者中，研究了对皮内注射活的普氏立克次体进行激发后局部血管周围单核细胞炎性浸润（PVI）的反应。在激发后6、24和48小时采集的皮肤打孔活检标本上，使用针对特异性单核细胞标志物的免疫组织化学方法。到24至48小时时，组织学结果与迟发型超敏反应（DTHR）一致。在48小时时，CD4+和CD8+ T淋巴细胞在PVI中占主导地位。CD8+细胞比CD4+细胞更快进入PVI。在免疫的人类受试者中，对普氏立克次体激发的局部控制与富含γ-干扰素来源的T淋巴细胞以及对伤寒杆菌感染细胞具有潜在细胞毒性的CD8+ T细胞募集到PVI中有关。
