Chulavatnatol M, Yindepit S
J Reprod Fertil. 1976 Sep;48(1):91-7. doi: 10.1530/jrf.0.0480091.
Rat spermatozoa from the cauda epididymidis were found to have a lower activity of the surface ATPase than the spermatozoa from the caput region. The enzyme from spermatozoa of both regions had the same Michaelis constant (Km) for ATP of 5 X 10(-4) M. It was partly inhibited by ouabain and fluoride, but strongly inhibited by Cu2+, Zn2+,p-chloromercuribenzoate, 8-anilino-1-naphthalenesulphonate Triton X-100, Lubrol-PX, urea, guanidine hydrochloride, sodium dodecyl sulphate and glycerylphosphorylcholine. The enzyme of the spermatozoa from the cauda epididymidis was more sensitive to inhibition by ouabain and fluoride but less sensitive to inhibition by Cu2+ than that of the cells form the caput region. The Arrhenius plot of the temperature dependence of enzymatic activity varied for the cells from the caput and cauda epididymidis. The differences in the enzyme properties of spermatozoa from the two regions of the epididymis suggested that the decline in the activity during epididymal maturation may reflect changes in the lipids and sulphydryl groups of the sperm membrane.
研究发现，来自附睾尾的大鼠精子的表面ATP酶活性低于头部区域的精子。两个区域精子的该酶对ATP的米氏常数（Km）均为5×10⁻⁴M。它部分受哇巴因和氟化物抑制，但强烈受Cu²⁺、Zn²⁺、对氯汞苯甲酸、8-苯胺基-1-萘磺酸盐、曲拉通X-100、月桂醇聚醚-9、尿素、盐酸胍、十二烷基硫酸钠和甘油磷酸胆碱抑制。附睾尾精子的酶对哇巴因和氟化物抑制更敏感，但对Cu²⁺抑制的敏感性低于头部区域的细胞。附睾头部和尾部细胞酶活性的温度依赖性的阿伦尼乌斯曲线有所不同。附睾两个区域精子的酶特性差异表明，附睾成熟过程中活性的下降可能反映了精子膜脂质和巯基的变化。
