Cotten M, Wagner E, Zatloukal K, Phillips S, Curiel D T, Birnstiel M L
Research Institute for Molecular Pathology, Vienna, Austria.
Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6094-8. doi: 10.1073/pnas.89.13.6094.
One limit to successful receptor-mediated gene delivery is the exit of the endocytosed material from the endosome. We demonstrate here the delivery of marker genes to tissue culture cells using a modification of the receptor-mediated gene delivery technique that exploits the endosomolytic activity of defective adenovirus particles. In particular, greater than 90% of the transfected-cell population is found to express a beta-galactosidase gene, and, most importantly, this high level of expression can be obtained with psoralen-inactivated virus particles. Furthermore, because the delivered gene is not carried within the genome of the adenovirus particle, the size constraints are relieved, and we can, therefore, show the delivery of a 48-kilobase cosmid DNA molecule.
受体介导的基因递送成功的一个限制因素是内吞物质从内体中释放出来。我们在此展示了通过对受体介导的基因递送技术进行改进，利用缺陷腺病毒颗粒的溶酶体活性，将标记基因递送至组织培养细胞。特别是，发现超过90%的转染细胞群体表达β-半乳糖苷酶基因，而且最重要的是，用补骨脂素灭活的病毒颗粒也能获得这种高水平的表达。此外，由于递送的基因不携带在腺病毒颗粒的基因组内，因此解除了大小限制，所以我们能够展示一个48千碱基的黏粒DNA分子的递送。
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