The effects of ethanol on intracellular potassium and the membrane potential of an identified crab motor axon.

1. Observations were made on the fast bender excitor axon in autotomized crab walking limbs bathed in normal crab saline, and in salines made up with 0.2 M sucrose or 0.2 M ethanol. Microelectrode techniques were used to measure the resting membrane potential and the intracellular level of potassium. 2. Sucrose-saline had little effect on the membrane potential or the intracellular level of potassium. Ethanol-saline hyperpolarized the membrane potential by about 3 mV and increased the level of intracellular potassium. 3. The ethanol-induced changes in intracellular potassium levels and membrane potential took place with the same time course. Further, the changes in membrane potential could be accounted for by changes in the equilibrium potential for potassium, Ek as predicted by the Nernst equation.