胰岛素样生长因子与火鸡卫星细胞和胚胎成肌细胞相互作用的比较。

Comparison of insulin-like growth factor interaction with satellite cells and embryonic myoblasts derived from the turkey.

Sun S S, McFarland D C, Ferrin N H, Gilkerson K K

Department of Animal and Range Science, South Dakota State University, Brookings 57007-0392.

Comp Biochem Physiol Comp Physiol. 1992 Jun;102(2):235-43. doi: 10.1016/0300-9629(92)90127-c.

Abstract

The interaction of insulin-like growth factors (IGFs) with receptors on clonal-derived turkey satellite cells and embryonic myoblasts was compared using competitive binding assays and affinity cross-linking analysis. 2. Although [125I]IGF-I and [125I]IGF-II were displaced similarly by IGF-I and IGF-II within cell lines (P greater than 0.05), displacement, and therefore dissociation constants, differed between cell lines (P less than 0.0001). 3. Receptor cross-linking analysis using iodinated IGFs suggests that both IGF-I and IGF-II interact with the type I receptor on turkey embryonic and posthatch myogenic cells.

摘要

使用竞争性结合试验和亲和交联分析比较了胰岛素样生长因子（IGFs）与克隆来源的火鸡卫星细胞和胚胎成肌细胞上受体的相互作用。2. 虽然在细胞系中，[125I]IGF-I和[125I]IGF-II被IGF-I和IGF-II类似地取代（P大于0.05），但不同细胞系之间的取代以及解离常数存在差异（P小于0.0001）。3. 使用碘化IGFs进行的受体交联分析表明，IGF-I和IGF-II均与火鸡胚胎和孵化后肌原细胞上的I型受体相互作用。