Makino M, Sei Y, Arora P K, Morse H C, Hartley J W
Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
J Immunol. 1992 Sep 1;149(5):1707-13.
After infection with LP-BM5 murine leukemia viruses, susceptible strains of mice develop a severe and progressive immunodeficiency disease, termed murine AIDS (MAIDS), features of which include markedly impaired T cell response to mitogens or specific Ag stimulation and decreased production of IL-2. Since an elevation of intracellular calcium concentration resulting from binding of Ag to the TCR is associated with IL-2 production, T cells from mice either uninfected or infected with LP-BM5 murine leukemia viruses were examined by a calcium mobilization assay. Both CD4+ and CD8+ T cells from infected mice manifested impaired calcium mobilization responses upon in vitro stimulation with anti-CD3 mAb or Con A. The abnormalities appeared early after virus inoculation and showed no difference in time course between subsets of T cells. Frequencies of prestimulation calcium-positive cells among both CD4+ and CD8+ cells in mice with MAIDS were significantly higher than those for uninfected mice. These abnormalities were associated with presence of the MAIDS-inducing defective virus genome, but were not induced by infection of mice genetically resistant to development of MAIDS or with nonpathogenic helper murine leukemia virus, a virus component that induces high spontaneous proliferation of T cells, even in MAIDS-resistant mice.
感染LP - BM5鼠白血病病毒后，易感品系的小鼠会发展出一种严重的进行性免疫缺陷疾病，称为鼠艾滋病（MAIDS），其特征包括T细胞对丝裂原或特异性抗原刺激的反应明显受损以及白细胞介素-2的产生减少。由于抗原与T细胞受体结合导致细胞内钙浓度升高与白细胞介素-2的产生相关，因此通过钙动员试验检测了未感染或感染LP - BM5鼠白血病病毒的小鼠的T细胞。感染小鼠的CD4 +和CD8 + T细胞在体外受到抗CD3单克隆抗体或刀豆蛋白A刺激时，均表现出钙动员反应受损。这些异常在病毒接种后早期出现，并且在T细胞亚群之间的时间进程上没有差异。患有MAIDS的小鼠的CD4 +和CD8 +细胞中刺激前钙阳性细胞的频率显著高于未感染小鼠。这些异常与诱导MAIDS的缺陷病毒基因组的存在有关，但不是由对MAIDS发展具有遗传抗性的小鼠感染或用非致病性辅助鼠白血病病毒感染诱导的，非致病性辅助鼠白血病病毒是一种即使在对MAIDS有抗性的小鼠中也能诱导T细胞高自发增殖的病毒成分。
