Liao N, Vaudry H, Pelletier G
MRC Group in Molecular Endocrinology, CHUL Research Center, Quebec, Canada.
Peptides. 1992 Jul-Aug;13(4):677-80. doi: 10.1016/0196-9781(92)90172-y.
In order to investigate the possible involvement of corticotropin-releasing factor (CRF) and somatostatin (SRIF) on thyrotropin-releasing hormone (TRH) neuronal cell activity in the rat hypothalamic paraventricular nucleus, we have proceeded to the simultaneous localization of CRF or SRIF and TRH. For this purpose, we used a dual immunostaining procedure that employed antibodies to CRF and SRIF and peroxidase-labeled goat anti-rabbit IgG as a first sequence, and antibodies to a cryptic fragment (Phe178-Glu199) of pro-TRH (to label TRH neurons) and alkaline phosphatase-labeled goat anti-rabbit IgG as the second sequence. A rich innervation of the paraventricular nucleus by immunoreactive CRF and SRIF fibers was observed. A large number of CRF and SRIF nerve endings were seen intimate anatomic proximity and often appeared to surround TRH-containing cell bodies. These results strongly suggest that TRH neurons might be regulated by both CRF and SRIF. These interactions might be the neuroanatomical basis for the already observed inhibitory effects of CRF and SRIF on TRH release.
为了研究促肾上腺皮质激素释放因子（CRF）和生长抑素（SRIF）对大鼠下丘脑室旁核促甲状腺激素释放激素（TRH）神经元细胞活性的可能影响，我们对CRF或SRIF与TRH进行了同步定位。为此，我们采用了双重免疫染色程序，第一步使用抗CRF和抗SRIF抗体以及过氧化物酶标记的山羊抗兔IgG，第二步使用抗前体TRH的隐蔽片段（Phe178-Glu199）抗体（用于标记TRH神经元）和碱性磷酸酶标记的山羊抗兔IgG。观察到免疫反应性CRF和SRIF纤维对室旁核有丰富的神经支配。大量的CRF和SRIF神经末梢在解剖学上紧密相邻，并且常常似乎围绕着含有TRH的细胞体。这些结果强烈表明，TRH神经元可能受CRF和SRIF两者的调节。这些相互作用可能是CRF和SRIF对TRH释放的抑制作用的神经解剖学基础。
