Hauser L, Carvalho G R, Hughes R N, Carter R E
Marine, Environmental and Evolutionary Research Group, School of Biological Sciences, University College of Swansea, U.K.
Proc Biol Sci. 1992 Jul 22;249(1324):19-25. doi: 10.1098/rspb.1992.0078.
Multi-locus DNA fingerprints were obtained from individuals of the hydrobiid snail, Potamopyrgus antipodarum (= P. jenkinsi), by using an RNA derivative (pSPT 18.15) of Jeffrey's 33.15 minisatellite core sequence. Whole-body homogenization of snails yielded 3.21 +/- 0.09 micrograms DNA per individual, producing complex profiles comprising 12-22 fragments within the 1.0-20.0 kilobase (kb) size range. Fingerprints from natural and experimental populations identified three distinct clonal genotypes corresponding to morphological strains A, B and C, with only rare mutational variants. Mother-offspring comparisons of genetic fingerprints revealed genetic stability during apomictic parthenogenesis. Data support the notion that British populations of P. antipodarum comprise three widespread obligate parthenogenetic clones resulting from a mid-19th Century introduction from Australasia. The present-day low levels of genotypic diversity are discussed in relation to the typical occurrence of P. antipodarum in man-made or immature habitats.
通过使用杰弗里氏33.15微卫星核心序列的一种RNA衍生物（pSPT 18.15），从新西兰泥蜗（Potamopyrgus antipodarum，又名詹氏新西兰泥蜗）个体中获得了多位点DNA指纹图谱。蜗牛的全身匀浆每个个体产生3.21±0.09微克DNA，产生了复杂的图谱，在1.0至20.0千碱基（kb）大小范围内包含12 - 22个片段。来自自然种群和实验种群的指纹图谱确定了三种不同的克隆基因型，分别对应形态学菌株A、B和C，只有罕见的突变变体。遗传指纹的母子比较揭示了无融合生殖孤雌生殖过程中的遗传稳定性。数据支持这样一种观点，即英国的新西兰泥蜗种群由三个广泛分布的专性孤雌生殖克隆组成，这些克隆源于19世纪中叶从澳大拉西亚引入。结合新西兰泥蜗在人造或未成熟栖息地的典型出现情况，讨论了当今基因型多样性水平较低的问题。
