亲代暴露于N-乙基-N-亚硝基脲诱导产生的一种胞质环氧化物水解酶变体形式的生化特性
Biochemical characterization of a variant form of cytosolic epoxide hydrolase induced by parental exposure to N-ethyl-N-nitrosourea.
作者信息
Nourooz-Zadeh J, Winder B S, Dietze E C, Giometti C S, Tollaksen S L, Hammock B D
机构信息
Department of Entomology, University of California, Davis 95616.
出版信息
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1992 Sep;103(1):207-14. doi: 10.1016/0742-8413(92)90253-4.
PMID:1360372
Abstract
- ENU4 mice express a protein variant originally detected in a CBF1 mouse sired by a C57BL/6 mouse exposed to N-ethyl-N-nitrosourea. It appears to be an isoelectric point variant of cytosolic epoxide hydrolase. Affinity purified cytosolic epoxide hydrolase from ENU4 mice has a pI of approximately 5.1 compared to 5.6 in other mouse strains. 2. Clofibrate induced cytosolic epoxide hydrolase to similar levels in five strains of mice. However, CBF1 and ENU4 mice were more sensitive to the induction of palmitoyl CoA oxidase activity. 3. Except for isoelectric point, the physico- and immunochemical properties of cytosolic epoxide hydrolase from ENU4 mice were similar to those of the other mouse strains. Substrate specificities for five of six substrates tested were also similar.
摘要
- ENU4小鼠表达一种蛋白质变体，最初是在一只由暴露于N-乙基-N-亚硝基脲的C57BL/6小鼠所生的CBF1小鼠中检测到的。它似乎是胞质环氧化物水解酶的等电点变体。与其他小鼠品系相比，从ENU4小鼠中亲和纯化的胞质环氧化物水解酶的pI约为5.1，而其他小鼠品系为5.6。2. 氯贝丁酯在五种小鼠品系中诱导胞质环氧化物水解酶达到相似水平。然而，CBF1和ENU4小鼠对棕榈酰辅酶A氧化酶活性的诱导更敏感。3. 除等电点外，ENU4小鼠胞质环氧化物水解酶的物理和免疫化学性质与其他小鼠品系相似。所测试的六种底物中的五种的底物特异性也相似。
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