亚精胺/精胺N1-乙酰基转移酶，大鼠膀胱上皮细胞增殖的一种新的生化标志物。
Spermidine/spermine N1-acetyltransferase, a new biochemical marker for epithelial proliferation in rat bladder.
作者信息
Matsui-Yuasa I, Otani S, Yano Y, Takada N, Shibata M A, Fukushima S
机构信息
Second Department of Biochemistry, Osaka City University Medical School.
出版信息
Jpn J Cancer Res. 1992 Oct;83(10):1037-40. doi: 10.1111/j.1349-7006.1992.tb02718.x.
We examined the activity of spermidine/spermine N1-acetyltransferase (SAT), a rate-limiting enzyme of the biodegradation of polyamines, in N-butyl-N-(4-hydroxybutyl)nitrosamine-induced transitional cell carcinoma (TCC) and melamine-induced papillomatosis of rat bladder, and compared the activity to that of ornithine decarboxylase (ODC). Both activities were higher in both lesions than in control rats. The difference between SAT and ODC activities in cancerous tissue and papillomatosis was not significant. Cells stained for proliferating cell nuclear antigen (PCNA) were abundant in papillomatosis. TCC had areas with much PCNA. The results indicated that an elevation of SAT activity occurs in both reversible and irreversible proliferation of bladder epithelium and could be important in bladder carcinogenesis.
我们检测了亚精胺/精胺N1-乙酰基转移酶（SAT）的活性，该酶是多胺生物降解的限速酶，在N-丁基-N-（4-羟丁基）亚硝胺诱导的大鼠膀胱移行细胞癌（TCC）和三聚氰胺诱导的大鼠膀胱乳头状瘤中进行检测，并将其活性与鸟氨酸脱羧酶（ODC）的活性进行比较。在这两种病变中，这两种酶的活性均高于对照大鼠。癌组织和乳头状瘤中SAT和ODC活性的差异不显著。乳头状瘤中增殖细胞核抗原（PCNA）染色阳性的细胞丰富。TCC中有许多PCNA阳性区域。结果表明，SAT活性升高发生在膀胱上皮的可逆性和不可逆性增殖中，并且在膀胱癌发生过程中可能起重要作用。
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