GRIFFITH T, BYERRUM R U
Science. 1959 May 29;129(3361):1485-6. doi: 10.1126/science.129.3361.1485.
Radioactive nicotine was synthesized by Nicotiana rustica L. which was fed sodium acetate-1-C(14), sodium acetate-2-C(14), sodium pyruvate-1-C(14), or sodium pyruvate-3-C(14). Acetate-2-C(14) and pyruvate-3-C(14) were converted to nicotine with the least dilution of radioisotope, whereas pyruvate-1-C(14) was incorporated with a relatively large dilution. When acetate-1-C(14) was administered to the plants the nicotine contained C(14) only in the pyrrolidine ring. After acetate-2-C(14) was fed, C(14) was located in both the pyrrolidine and pyridine rings.
放射性尼古丁由黄花烟草（Nicotiana rustica L.）合成，该烟草被喂食乙酸钠-1-C(14)、乙酸钠-2-C(14)、丙酮酸钠-1-C(14)或丙酮酸钠-3-C(14)。乙酸-2-C(14)和丙酮酸盐-3-C(14)转化为尼古丁时放射性同位素稀释最少，而丙酮酸盐-1-C(14)掺入时稀释相对较大。当向植物施用乙酸-1-C(14)时，尼古丁中的C(14)仅存在于吡咯烷环中。喂食乙酸-2-C(14)后，C(14)位于吡咯烷环和吡啶环中。
