粗糙脉孢菌中基因改造的色氨酸合成酶的酶活性。

Enzymatic activity of a genetically altered tryptophan synthetase in Neurospora crassa.

作者信息

SUSKIND S R, JORDAN E

出版信息

Science. 1959 Jun 12;129(3363):1614-5. doi: 10.1126/science.129.3363.1614.

DOI:10.1126/science.129.3363.1614

Abstract

Partially purified preparations of certain tryptophan-requiring mutants of Neurospora crassa, which contain a protein (CRM) antigenically related to wild-type tryptophan synthetase, possess indole glycerol phosphate-synthesizing activity. This activity can be inhibited by anti-CRM sera. It is suggested that CRM in such mutants represents a damaged tryptophan synthetase lacking the capacity to react with l-serine.

摘要

粗糙脉孢菌某些需要色氨酸的突变体的部分纯化制剂，含有一种与野生型色氨酸合成酶在抗原性上相关的蛋白质（CRM），具有吲哚甘油磷酸合成活性。这种活性可被抗CRM血清抑制。有人提出，此类突变体中的CRM代表一种受损的色氨酸合成酶，缺乏与L-丝氨酸反应的能力。