用口蹄疫病毒感染性核糖核酸进行密度梯度离心
Densitygradient centrifugation with infectious ribonucleic acid of foot-and-mouth disease virus.
作者信息
STROHMAIER K, MUSSGAY M
出版信息
Science. 1959 Jul 24;130(3369):217. doi: 10.1126/science.130.3369.217.
PMID:13668550
Abstract
The sedimentation constant of an infectious component in ribonucleic acid preparations from foot-and-mouth disease virus has been determined by density-gradient centrifugation. A sedimentation constant of 37 Svedberg units (S(t)) was obtained. On the assumption that the relation between the molecular weight and the sedimentation constant found by Gierer is applicable to our system as well, a value of 3.1 x 10(6) was calculated for the molecular weight of the infectious component.
摘要
通过密度梯度离心法测定了口蹄疫病毒核糖核酸制剂中感染性成分的沉降常数。得到的沉降常数为37斯维德伯格单位（S(t)）。假设吉勒发现的分子量与沉降常数之间的关系也适用于我们的体系，则计算出感染性成分的分子量为3.1×10⁶。
相似文献
1
Densitygradient centrifugation with infectious ribonucleic acid of foot-and-mouth disease virus.
Science. 1959 Jul 24;130(3369):217. doi: 10.1126/science.130.3369.217.
2
3
[Infectiousness of ribonucleic acid fraction of foot & mouth disease virus].
Experientia. 1959 Apr 15;15(4):155. doi: 10.1007/BF02165537.
4
Optimum conditions for the partial fractionation by ultra-centrifugation of guinea pig lymph diseased with foot-and-mouth virus into infectious and complement fixing components.
Acta Pathol Microbiol Scand. 1950;27(6):902-6. doi: 10.1111/j.1699-0463.1950.tb00092.x.
5
Thermal degradation of foot-and-mouth disease virus into infectious ribonucleic acid.
Proc Soc Exp Biol Med. 1961 Jul;107:610-3. doi: 10.3181/00379727-107-26703.
6
Infectivity of ribonucleic acid from mice and tissue culture infected with the virus of foot-and-mouth disease.
Nature. 1958 Aug 23;182(4634):535-6. doi: 10.1038/182535a0.
7
Large-scale production of bovine kidney cultures for plaque assay of foot-and-mouth disease virus and its ribonucleic acid.
Am J Vet Res. 1962 May;23:608-13.
8
The determination of the sedimentation constant of a homogeneous component having the characteristics of the foot- and mouth disease virus.
Am J Vet Res. 1952 Jan;13(46):13-6.
9
Studies with infective ribonucleic acid from tissues and cell cultures infected with the virus of foot-and-mouth disease.
Virology. 1959 Apr;7(4):408-18. doi: 10.1016/0042-6822(59)90069-8.
10
Ribonucleic acid of foot-and-mouth disease virus: its preparation, stability, and plating efficiency on bovine-kidney cultures.
Virology. 1960 Oct;12:258-71. doi: 10.1016/0042-6822(60)90199-9.
引用本文的文献
1
Physicochemical studies of Newcastle disease virus. 3. The content of virus nucleic acid and its sedimentation pattern.
Arch Gesamte Virusforsch. 1967;20(3):287-95. doi: 10.1007/BF01241948.
2
The nucleic acid of the Bryan strain of Rous sarcoma virus: purification of the virus and isolation of the nucleic acid.
Proc Natl Acad Sci U S A. 1965 Jul;54(1):137-44. doi: 10.1073/pnas.54.1.137.
Zotero 插件