Iwalokun B A, Gbenle G O, Ogunledun A
Biochemistry Department, College of Medicine, University of Lagos, PMB 12003, Nigeria.
J Health Popul Nutr. 2003 Jun;21(2):90-5.
Forty-five strains of Shigella were screened for haemagglutinin production and broad-spectrum haemagglutination reaction. Mannose-sensitive haemagglutinin (MSHA) was found in 22 strains [Shigella flexneri (7), S. dysenteriae (7), S. sonnei (3), and S. boydii (5)]. Eighteen strains harboured mannose-resistant haemagglutinin (MRHA), and 8 strains were observed to be non-haemagglutinating to guinea pig erythrocyte. With the exception of human erythrocytes (O, A, B, and AB), the observed MSHA and MRHA also agglutinated the erythrocytes of rabbit, sheep, rat, chicken, and horse, suggesting a broad-spectrum haemagglutinating property. Haemagglutinins of S. flexneri and S. dysenteriae elicited a relatively stronger haemagglutinating activity with agglutinability to chicken and rabbit erythrocytes enhanced by trypsinization. Haemagglutination reaction with guinea pig erythrocyte was generally inhibited by sialic acid, while simple sugars, such as D-glucose, D-galactose, N-acetylgalactosamine, N-acetylglucosamine, and D-rhamnose, elicited no inhibitory effect. The results of the study revealed broad-spectrum haemagglutinin expression by circulating Shigella strains in Nigeria.
对45株志贺氏菌进行了血凝素产生和广谱血凝反应的筛选。在22株菌中发现了甘露糖敏感血凝素（MSHA）[福氏志贺氏菌（7株）、痢疾志贺氏菌（7株）、宋内志贺氏菌（3株）和鲍氏志贺氏菌（5株）]。18株菌含有甘露糖抗性血凝素（MRHA），8株菌对豚鼠红细胞无凝集作用。除人红细胞（O、A、B和AB型）外，观察到的MSHA和MRHA还能凝集兔、羊、大鼠、鸡和马的红细胞，表明具有广谱血凝特性。福氏志贺氏菌和痢疾志贺氏菌的血凝素引发的血凝活性相对较强，胰蛋白酶处理可增强对鸡和兔红细胞的凝集能力。豚鼠红细胞的血凝反应一般受唾液酸抑制，而D-葡萄糖、D-半乳糖、N-乙酰半乳糖胺、N-乙酰葡糖胺和D-鼠李糖等单糖无抑制作用。研究结果显示尼日利亚循环志贺氏菌菌株具有广谱血凝素表达。
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