通过使用“全鱼”嵌合生长激素基因构建体提高转基因大西洋鲑的生长速度。

Growth enhancement in transgenic Atlantic salmon by the use of an "all fish" chimeric growth hormone gene construct.

作者信息

Du S J, Gong Z Y, Fletcher G L, Shears M A, King M J, Idler D R, Hew C L

机构信息

Research Institute, Hospital for Sick Children, Toronto, Canada.

出版信息

Biotechnology (N Y). 1992 Feb;10(2):176-81. doi: 10.1038/nbt0292-176.

DOI:10.1038/nbt0292-176

PMID:1368229

Abstract

We have developed an "all fish" growth hormone (GH) chimeric gene construct by using an antifreeze protein gene (AFP) promoter from ocean pout linked to a chinook salmon GH cDNA clone. After microinjection into fertilized, nonactivated Atlantic salmon eggs via the micropyle, transgenic Atlantic salmon were generated. The presence of the transgene was detected by polymerase chain reaction (PCR) using specific oligonucleotide primers. A number of these transgenic fish showed dramatic increases in their growth rate. At one year old, the average increase of the transgenic fish was 2 to 6 fold and the largest transgenic fish was 13 times that of the average non-transgenic control.

摘要

我们通过将来自海洋杜父鱼的抗冻蛋白基因（AFP）启动子与奇努克鲑鱼生长激素cDNA克隆相连，构建了一种“全鱼”生长激素（GH）嵌合基因结构。通过珠孔将其显微注射到未激活的受精大西洋鲑鱼卵中后，产生了转基因大西洋鲑鱼。使用特异性寡核苷酸引物通过聚合酶链反应（PCR）检测转基因的存在。许多这些转基因鱼的生长速度显著提高。在一岁时，转基因鱼的平均生长速度提高了2至6倍，最大的转基因鱼是平均非转基因对照鱼的13倍。