泡罩塔生物反应器中固定化棒曲霉细胞分批和连续生产核糖核酸酶

Batch and continuous ribonuclease production by immobilized Aspergillus clavatus cells in a bubble-column bioreactor.

作者信息

Manolov R J

机构信息

Department of Enzymes, Bulgarian Academy of Sciences, Sofia.

出版信息

Appl Microbiol Biotechnol. 1992 Apr;37(1):32-6. doi: 10.1007/BF00174199.

DOI:10.1007/BF00174199

PMID:1368500

Abstract

Ribonuclease production using immobilized cells (IC) of Aspergillus clavatus has been studied under batch, repeated-batch and continuous fermentation conditions in a bubble-column bioreactor and compared with production by free cells. Immobilization was achieved by the method of cryostructurization in polyvinyl alcohol beads. The effect of various aeration rates in a column bioreactor has been investigated. Enzyme production by IC [42,000 units (U).1(-1)] during batch fermentations was comparable to that of a free-cell system. The specific productivity of IC was 8.5 times higher than that of free cells. In repeated batch fermentation at various aeration rates, successful reuse of IC was obtained, with comparable levels of enzyme production. Continuous ribonuclease production was achieved for 44 days at 1 vvm aeration and a dilution rate of 0.01 h-1 with high volumetric productivity (450 U.1-1.h-1) and yield.

摘要

在鼓泡塔生物反应器中，对固定化棒曲霉细胞（IC）在分批、重复分批和连续发酵条件下生产核糖核酸酶进行了研究，并与游离细胞生产进行了比较。通过在聚乙烯醇珠粒中进行冷冻结构化方法实现固定化。研究了柱式生物反应器中不同通气速率的影响。在分批发酵过程中，固定化细胞生产的酶[42,000单位（U）·L⁻¹]与游离细胞系统相当。固定化细胞的比生产率比游离细胞高8.5倍。在不同通气速率下的重复分批发酵中，固定化细胞成功实现了重复使用，酶产量相当。在通气量为1 vvm、稀释率为0.01 h⁻¹的条件下，连续生产核糖核酸酶44天，具有较高的体积生产率（450 U·L⁻¹·h⁻¹）和产率。