Bach F H
Ann Allergy. 1976 Nov;37(5):307-27.
Two tests, the mixed leukocyte culture (MLC) and cell-mediated lympholysis (CML) tests, have been used as in vitro models of the in vivo allograft reaction. These tests have been applied to histocompatibility testing for transplantation as well as to assay of immune function. They involve the use of peripheral blood lymphocytes as "responding" cells in mixed leukocyte culture or as "stimulating" cells in the generation of a proliferative or a cytotoxic response. The proliferative events in MLC are primarily in response to LD antigens of the major histocompatibility complex; the cytotoxic cells use as their targets the SD antigens of that complex. A new method for defining the LD antigens, the primed LD typing (PLT) test, is based on in vitro sensitization of lymphocytes to certain LD antigens of the major histocompatibility complex and their subsequent restimulation with test cells.
混合淋巴细胞培养（MLC）和细胞介导的淋巴细胞溶解（CML）这两种试验，已被用作体内同种异体移植反应的体外模型。这些试验已应用于移植的组织相容性检测以及免疫功能测定。它们涉及在混合淋巴细胞培养中使用外周血淋巴细胞作为“反应”细胞，或在产生增殖或细胞毒性反应中作为“刺激”细胞。MLC中的增殖事件主要是对主要组织相容性复合体的LD抗原作出反应；细胞毒性细胞将该复合体的SD抗原作为其靶标。一种定义LD抗原的新方法，即致敏LD分型（PLT）试验，是基于淋巴细胞在体外对主要组织相容性复合体的某些LD抗原致敏，以及随后用测试细胞对其进行再次刺激。
