可溶性葡聚糖-磷酸腺苷复合物的合成与表征：辅酶负载的动力学效应

Synthesis and characterization of soluble dextran-adenosine phosphate complexes: kinetic effects of coenzyme loading.

作者信息

Hubble J, Eisenthal R, Herbert A, Tarr I, England R

机构信息

School of Chemical Engineering, University of Bath, UK.

出版信息

Enzyme Microb Technol. 1990 Mar;12(3):196-203. doi: 10.1016/0141-0229(90)90038-r.

DOI:10.1016/0141-0229(90)90038-r

PMID:1369982

Abstract

Soluble dextran-ATP complexes have been synthesized using a bifunctional oxirane as the coupling agent. The degree of coupling is time-dependent, allowing materials of varying coenzyme loadings to be produced very simply. Characterization studies have shown that at the maximum coenzyme loading obtained (34 molecules per complex) all coenzyme moieties were coenzymically active with hexokinase. The extent of coenzyme loading was shown to have a considerable influence on the values of Km and Vmax of the complex as a substrate for hexokinase. Enzyme activity was also found with acetate kinase and myokinase, and coenzyme recycling (ATP, ADP) was demonstrated in an ultrafiltration reactor.

摘要

已使用双功能环氧乙烷作为偶联剂合成了可溶性葡聚糖 - ATP 复合物。偶联程度与时间相关，这使得能够非常简单地制备具有不同辅酶负载量的材料。表征研究表明，在所获得的最大辅酶负载量下（每个复合物 34 个分子），所有辅酶部分对己糖激酶均具有辅酶活性。结果表明，辅酶负载程度对该复合物作为己糖激酶底物时的 Km 和 Vmax 值有相当大的影响。还发现该复合物对乙酸激酶和肌激酶也有酶活性，并且在超滤反应器中证明了辅酶循环（ATP、ADP）。