Narisawa Y, Hashimoto K, Bayless T, Nihei Y, Ishihara M, Lawrence D, Eto H, Hori K
Department of Dermatology and Syphilology, Wayne State University School of Medicine, Detroit, MI 48201.
J Invest Dermatol. 1992 Feb;98(2):171-80. doi: 10.1111/1523-1747.ep12555813.
The origin of Merkel cells is uncertain, although current evidence by immunohistochemical keratin marker studies favors an epidermal derivation. We studied the expression of keratin species in Merkel cells of human fetus and adult using 19 anti-keratin antibodies. Epidermal and dermal Merkel cells contained not only simple epithelium-type but also some stratified epithelium-type keratins. Interestingly, expression of some keratins was different between epidermal and dermal Merkel cells, for example, AN3 (50, 58 kD) and CKB1 (50 kD) recognized epidermal Merkel cells, but not dermal Merkel cells. These results suggest that surrounding keratinocytes influence the expression of cytokeratins in Merkel cells or that dermal Merkel cells undergo a modification from keratin-producing epidermal Merkel cells to a more neural cell type by the association with nerve endings in the upper dermis. On the other hand, certain cytokeratin polypeptides recognizable with Ks19.1 (40 kD), CK5 (45 kD), and CAM5.2 (52.5 kD) were expressed in both epidermal and dermal Merkel cells. The expression of simple epithelium-type keratins in Merkel cells remained even after the epidermal basal cells gradually lost their expression.
默克尔细胞的起源尚不确定，尽管目前免疫组化角蛋白标志物研究的证据支持其来源于表皮。我们使用19种抗角蛋白抗体研究了人类胎儿和成人默克尔细胞中角蛋白种类的表达。表皮和真皮中的默克尔细胞不仅含有简单上皮型角蛋白，还含有一些复层上皮型角蛋白。有趣的是，表皮和真皮默克尔细胞中某些角蛋白的表达有所不同，例如，AN3（50、58kD）和CKB1（50kD）可识别表皮默克尔细胞，但不能识别真皮默克尔细胞。这些结果表明，周围的角质形成细胞影响默克尔细胞中细胞角蛋白的表达，或者真皮默克尔细胞通过与真皮上层的神经末梢结合，从产生角蛋白的表皮默克尔细胞转变为更具神经细胞特征的细胞类型。另一方面，用Ks19.1（40kD）、CK5（45kD）和CAM5.2（52.5kD）可识别的某些细胞角蛋白多肽在表皮和真皮默克尔细胞中均有表达。即使表皮基底细胞逐渐失去其表达后，默克尔细胞中简单上皮型角蛋白的表达仍持续存在。
