Heffelfinger S C, Hawkins H H, Barrish J, Taylor L, Darlington G J
University of Cincinnati College of Medicine, Department of Pathology and Laboratory Medicine, Ohio 45267.
In Vitro Cell Dev Biol. 1992 Feb;28A(2):136-42. doi: 10.1007/BF02631017.
SK-HEP-1 is an immortal, human cell line derived from the ascitic fluid of a patient with adenocarcinoma of the liver. We have determined that these cells are of endothelial origin. Despite the location of the tumor from which SK HEP-1 was derived, the cell line does not have properties of hepatocytes. Northern blot analysis of total cellular RNA shows no messenger RNA for the hepatic-specific proteins albumin, alpha-fibrinogen, or gamma-fibrinogen. Endothelial characteristics are seen by transmission electron microscopy. These features include numerous pinocytotic vesicles, electron dense granules consistent with Weibel-Palade bodies, and abundant intermediate filaments, identified immunocytochemically as vimentin. Cultures grown on plastic dishes grow in bundles of polygonal to spindle-shaped cells. Proteins characteristic for endothelial cells are identified by immunocytochemistry. Addition of basement membrane material (Matrigel) or type I collagen to the cultures induces these cells to organize into a tubular network.
SK-HEP-1是一种永生的人细胞系，源自一名肝癌患者的腹水。我们已确定这些细胞起源于内皮细胞。尽管SK-HEP-1来源于肿瘤的位置，但该细胞系不具有肝细胞的特性。对总细胞RNA进行的Northern印迹分析显示，没有肝特异性蛋白白蛋白、α-纤维蛋白原或γ-纤维蛋白原的信使RNA。通过透射电子显微镜观察到内皮细胞特征。这些特征包括大量的胞饮小泡、与魏贝尔-帕拉德小体一致的电子致密颗粒，以及丰富的中间丝，经免疫细胞化学鉴定为波形蛋白。在塑料培养皿上生长的培养物中，细胞呈多边形至纺锤形的束状生长。通过免疫细胞化学鉴定出内皮细胞特有的蛋白质。向培养物中添加基底膜材料（基质胶）或I型胶原可诱导这些细胞组织成管状网络。
