Hurd Y L, Brown E E, Finlay J M, Fibiger H C, Gerfen C R
Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, MD 20892.
Brain Res Mol Brain Res. 1992 Mar;13(1-2):165-70. doi: 10.1016/0169-328x(92)90058-j.
The influence of cocaine self-administration on the expression of messenger RNAs for dynorphin, enkephalin and substance P was analyzed in the rat striatum with in situ hybridization histochemistry. Cocaine, an indirect dopamine agonist, was found to differentially affect the levels of mRNA encoding these neuropeptides in different subregions of the striatum. Following a 7 day period of variable free access to cocaine, dynorphin and substance P mRNA levels were elevated throughout the striatum, but the increases were substantially greater in the dorsal striatum than in the nucleus accumbens. Enkephalin mRNA was not significantly altered in the dorsal striatum but was slightly elevated in the nucleus accumbens. These results suggest that cocaine self-administration has differential effects on striatonigral and striatopallidal projection neurons, and that these effects vary in subregions of the striatum.
采用原位杂交组织化学技术，分析了可卡因自身给药对大鼠纹状体中强啡肽、脑啡肽和P物质信使核糖核酸（mRNA）表达的影响。可卡因作为一种间接多巴胺激动剂，被发现可不同程度地影响纹状体不同亚区中编码这些神经肽的mRNA水平。在给予7天可变自由获取可卡因的时间段后，整个纹状体中强啡肽和P物质的mRNA水平均升高，但背侧纹状体中的升高幅度明显大于伏隔核。脑啡肽mRNA在背侧纹状体中无显著变化，但在伏隔核中略有升高。这些结果表明，可卡因自身给药对黑质纹状体和纹状体苍白球投射神经元有不同影响，且这些影响在纹状体的亚区中有所不同。
