从豚鼠子宫中克隆P物质受体cDNA
Molecular cloning of substance P receptor cDNA from guinea-pig uterus.
作者信息
Gorbulev V, Akhundova A, Luzius H, Fahrenholz F
机构信息
Max-Planck-Institute für Biophysik, Frankfurt, Germany.
出版信息
Biochim Biophys Acta. 1992 May 7;1131(1):99-102. doi: 10.1016/0167-4781(92)90105-9.
A cDNA encoding guinea-pig uterine substance P (SP) receptor has been isolated using the homology screening approach. Northern blot analysis reveals that the corresponding mRNA, of approx. 4.8 kb, is expressed in all tissues tested, but predominantly in the uteri of non-pregnant animals; during pregnancy its expression is reduced. The guinea-pig SP receptor was expressed in COS-7 cells and demonstrated relative ligand affinity in the order: SP much greater than neurokinin A greater than neurokinin B.
利用同源筛选方法分离出了一种编码豚鼠子宫P物质（SP）受体的互补DNA（cDNA）。Northern印迹分析显示，相应的信使核糖核酸（mRNA）约4.8千碱基对（kb），在所检测的所有组织中均有表达，但在未孕动物的子宫中表达量最高；在怀孕期间其表达量降低。豚鼠SP受体在COS-7细胞中表达，并显示出相对配体亲和力顺序为：SP远大于神经激肽A大于神经激肽B。
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