van der Hoorn F A
University of Calgary, Department of Medical Biochemistry, Alberta, Canada.
Oncogene. 1992 Jun;7(6):1093-9.
The c-mos proto-oncogene is predominantly expressed in male and female germ cells and is involved in the regulation of meiosis. To investigate the mechanism of testis-specific regulation of c-mos transcription, I set out to identify the rat testis c-mos promoter. This was achieved by characterization of the rat testis c-mos transcription start site by primer extension and sequence analysis of cDNAs obtained by polymerase chain reaction amplification of 5' ends of c-mos RNA. The rat testis c-mos transcription start site is located 0.56 kb upstream of the coding region. A fragment containing the rat testis c-mos promoter directs transcription in a nuclear extract derived from rat seminiferous tubules, but not in a liver nuclear extract. DNAase I footprint analysis and gel-retardation assays showed binding of a novel testis-specific nuclear factor to the rat testis c-mos promoter at a site homologous to the testis-specific cis-acting element identified in the promoter of the RT7 gene, which is specifically expressed in haploid male germ cells.
原癌基因c-mos主要在雄性和雌性生殖细胞中表达，并参与减数分裂的调控。为了研究c-mos转录的睾丸特异性调控机制，我着手鉴定大鼠睾丸c-mos启动子。这是通过引物延伸法对大鼠睾丸c-mos转录起始位点进行表征，并对通过聚合酶链反应扩增c-mos RNA 5'端获得的cDNA进行序列分析来实现的。大鼠睾丸c-mos转录起始位点位于编码区上游0.56 kb处。一个包含大鼠睾丸c-mos启动子的片段在源自大鼠生精小管的核提取物中指导转录，但在肝核提取物中则不然。DNA酶I足迹分析和凝胶阻滞试验表明，一种新的睾丸特异性核因子在与RT7基因启动子中鉴定的睾丸特异性顺式作用元件同源的位点与大鼠睾丸c-mos启动子结合，RT7基因在单倍体雄性生殖细胞中特异性表达。
