Learn D B, Moloney S J, Giddens L D
Skin Biology Research, Schering-Plough HealthCare Products Inc., Memphis, Tennessee 38151.
Biotech Histochem. 1992 May;67(3):125-30. doi: 10.3109/10520299209110021.
We compared three different staining methods to determine if the dermal elastic fiber content of the HRS/Skh-1 hairless mouse could be accurately measured by color image analysis. Comparisons were made among Kligman's modification of Luna's mast cell stain for elastin, Unna's orcein stain with or without potassium permanganate preoxidation, and Gomori's aldehyde fuchsin stain with potassium permanganate preoxidation. The color image analysis system could be used to identify and quantify murine dermal elastin fibers in sections stained by all three methods. Gomori's aldehyde fuchsin stain with preoxidation demonstrated twice the content of dermal elastic fibers demonstrated by either Kligman's modification of Luna's mast cell stain or Unna's orcein stain with or without preoxidation. Gomori's aldehyde fuchsin method with preoxidation should be considered the stain of choice for evaluating murine dermal elastic fiber content.
我们比较了三种不同的染色方法，以确定能否通过彩色图像分析准确测量HRS/Skh-1无毛小鼠的真皮弹性纤维含量。对用于弹性蛋白的Kligman改良Luna肥大细胞染色法、经或未经高锰酸钾预氧化的Unna地衣红染色法以及经高锰酸钾预氧化的Gomori醛复红染色法进行了比较。彩色图像分析系统可用于识别和量化用这三种方法染色的切片中的小鼠真皮弹性纤维。经预氧化的Gomori醛复红染色法显示的真皮弹性纤维含量是Kligman改良Luna肥大细胞染色法或经或未经预氧化的Unna地衣红染色法所显示含量的两倍。经预氧化的Gomori醛复红法应被视为评估小鼠真皮弹性纤维含量的首选染色方法。
