Qiao J H, Ruan X M, Trento A, Czer L S, Blanche C, Fishbein M C
Division of Anatomic Pathology, Cedars-Sinai Medical Center, Los Angeles, Calif. 90048.
J Heart Lung Transplant. 1992 Sep-Oct;11(5):920-5.
Adhesion of leukocytes to vascular endothelial cells is a critical step in a variety of inflammatory conditions. We studied the expression and distribution of intercellular adhesion molecule-1 (ICAM-1) and endothelial leukocyte adhesion molecule-1 (ELAM-1) in frozen sections of 83 endomyocardial biopsy specimens from human allograft hearts using monoclonal antibodies and an avidin-biotin complex-alkaline phosphatase staining technique. Cases with cellular or humoral rejection and Quilty lesions were studied. Staining was graded from 0 to 3+ in lymphocytes and in capillary, arterial, venular, and endocardial endothelial cells. Expression of ICAM-1 in capillaries increased with the severity of cellular rejection and was prominent in humoral rejection. ICAM-1 was also expressed in lymphocytes in proportion to the degree of rejection. Little or no ELAM-1 expression was noted. In Quilty lesions the intensity of ICAM-1 expression was similar to that of mild-to-moderate rejection. Thus adhesion molecule expression can be identified in endomyocardial biopsy specimens of patients with rejection, suggesting a role for adhesion molecules in the process of rejection. These findings may prove useful in monitoring rejection and its response to therapy and in developing specific antisera directed against these molecules.
白细胞与血管内皮细胞的黏附是多种炎症状态下的关键步骤。我们使用单克隆抗体和抗生物素蛋白-生物素复合物-碱性磷酸酶染色技术，研究了取自人类同种异体移植心脏的83份心内膜心肌活检标本冰冻切片中细胞间黏附分子-1（ICAM-1）和内皮白细胞黏附分子-1（ELAM-1）的表达及分布。对出现细胞或体液排斥反应以及奎尔蒂病变的病例进行了研究。淋巴细胞以及毛细血管、动脉、静脉和心内膜内皮细胞的染色从0级到3+级进行分级。毛细血管中ICAM-1的表达随着细胞排斥反应的严重程度增加而升高，在体液排斥反应中尤为显著。ICAM-1在淋巴细胞中的表达也与排斥程度成正比。几乎未观察到ELAM-1的表达。在奎尔蒂病变中，ICAM-1的表达强度与轻度至中度排斥反应相似。因此，在排斥反应患者的心内膜心肌活检标本中可以识别黏附分子的表达，这表明黏附分子在排斥反应过程中发挥作用。这些发现可能在监测排斥反应及其对治疗的反应以及开发针对这些分子的特异性抗血清方面具有重要意义。
