Matsumoto Y, Ueda S, Kawata M
Department of Anatomy, Kyoto Prefectural University of Medicine, Japan.
Brain Res Dev Brain Res. 1992 Oct 23;69(2):153-65. doi: 10.1016/0165-3806(92)90154-o.
Fetal rat spinal cord transplanted into the anterior chamber of the eye of an adult rat was immunohistochemically stained using antisera to substance P (SP), neuropeptide Y (NPY), methionine-enkephalin (ENK), vasoactive intestinal polypeptide (VIP), calcitonin gene-related peptide (CGRP) and tyrosine hydroxylase (TH), and distributional changes of peptide- and enzyme-containing neurons 1, 2 and 4 weeks after transplantation were investigated. To examine the effect of colchicine on immunoreactivity, unilateral eyes of these adult host rats received intraocular colchicine treatment. Without colchicine treatment, numerous SP- and CGRP-immunoreactive (IR) neurons were observed in the graft 1 week after transplantation, and their immunoreactivity gradually decreased up to 4 weeks after transplantation. NPY-, ENK-and VIP-IR neurons first appeared in the graft 2 weeks after transplantation. Four weeks after transplantation, the immunoreactivity of NPY and ENK decreased significantly, whereas VIP-IR neurons showed the same intensity as that observed at 2 weeks after transplantation. TH-IR neurons, on the other hand, were seen at every stage, but their immunoreactivity was constant all the time. After colchicine treatment, the number of SP-, NPY-, ENK- and CGRP-IR neurons appeared to increase, while that of VIP- and TH-IR neurons did not change significantly. The distribution patterns of the peptide- and enzyme-containing fibers differed from each other. In the analysis of serial sections stained with 5 peptides (SP, NPY, ENK, VIP, CGRP), fibers containing these peptides were found to be densely accumulated in specific areas of the transplanted spinal cord. The present findings demonstrated that most of the peptide- and enzyme-containing neuron systems in the transplanted spinal cord showed similar distribution patterns and development to those in the normal spinal cord, but that some displayed different distribution.
将胎鼠脊髓移植到成年大鼠眼的前房，使用抗P物质（SP）、神经肽Y（NPY）、甲硫氨酸脑啡肽（ENK）、血管活性肠多肽（VIP）、降钙素基因相关肽（CGRP）和酪氨酸羟化酶（TH）的抗血清进行免疫组织化学染色，并研究移植后1、2和4周含肽和含酶神经元的分布变化。为了检测秋水仙碱对免疫反应性的影响，对这些成年宿主大鼠的单侧眼睛进行眼内秋水仙碱处理。未经秋水仙碱处理时，移植后1周在移植物中观察到大量SP和CGRP免疫反应性（IR）神经元，其免疫反应性在移植后4周逐渐降低。NPY、ENK和VIP-IR神经元在移植后2周首次出现在移植物中。移植后4周，NPY和ENK的免疫反应性显著降低，而VIP-IR神经元显示出与移植后2周观察到的强度相同。另一方面，TH-IR神经元在每个阶段都可见，但其免疫反应性一直恒定。秋水仙碱处理后，SP、NPY、ENK和CGRP-IR神经元的数量似乎增加，而VIP和TH-IR神经元的数量没有显著变化。含肽和含酶纤维的分布模式彼此不同。在用5种肽（SP、NPY、ENK、VIP、CGRP）染色的连续切片分析中，发现含有这些肽的纤维密集聚集在移植脊髓的特定区域。本研究结果表明，移植脊髓中大多数含肽和含酶的神经元系统显示出与正常脊髓相似的分布模式和发育情况，但有些显示出不同的分布。
