探寻胰凝乳蛋白酶活性中心的早期实验。
Early experiments in the quest of an active center in chymotrypsin.
作者信息
BALLS A K
出版信息
J Gen Physiol. 1962 Mar;45(4)Pt 2(4):47-56. doi: 10.1085/jgp.45.4.47.
Abstract
It is now quite proper to speak of an "active site" or "center" in (or on) a number of hydrolytic enzymes, chymotrypsin in particular. Such sites have been clearly demonstrated and in some cases they have even been investigated in detail. We are almost persuaded that they are the actual loci of enzyme activity in nature. Whatever we think of them, we do know that they are "some peculiar arrangement of amino acids" (2 a).
摘要
现在提及许多水解酶，尤其是胰凝乳蛋白酶中（或表面）的“活性部位”或“中心”是完全恰当的。这些部位已得到明确证实，在某些情况下甚至已进行了详细研究。我们几乎相信它们就是酶在自然状态下的实际活性位点。无论我们对它们作何看法，我们确实知道它们是“氨基酸的某种特殊排列”（2a）。
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