Tucková L, Rejnek J, Zikán J, Vĕtvicka V
Department of Immunology, Czechoslovak Academy of Sciences, Prague.
Folia Microbiol (Praha). 1992;37(1):71-8. doi: 10.1007/BF02814584.
The rosettes formed by mouse peritoneal macrophages or DCH-5 cells and TNP-erythrocytes coated with anti-TNP antibodies of different isotypes were inhibited to various extent by monosaccharides. The most effective inhibitors were N-acetylglucosamine, glucosamine, mannose and N-acetylneuraminic acid in 1-5 mmol/L concentrations. Even more efficient were glycopeptides isolated from IgG molecules. The Fc receptors (FcRs) released from DCH-5 cells during cultivation and gradually separated by affinity chromatography on immobilized IgG reacted with aggregated IgG and inhibited the rosette formation. The FcRs eluted by monosaccharides influenced mainly the number of rosettes mediated by IgA and IgE while those eluted with a glycine-HCl buffer inhibited preferentially IgG rosettes. As shown by SDS-PAGE the heterogeneity of the fraction eluted with a mixture of monosaccharides revealed one main component with an effective molar mass of 50 kg/mol. The glycine-HCl eluate contained two major components of 55 and 38 kg/mol. The IgG-Sepharose 4B bound all the fractions but only the binding of the 50 kg/mol molecule could be inhibited by monosaccharides.
由小鼠腹腔巨噬细胞或DCH - 5细胞与用不同同种型抗TNP抗体包被的TNP - 红细胞形成的玫瑰花结，受到单糖不同程度的抑制。最有效的抑制剂是浓度为1 - 5 mmol/L的N - 乙酰葡糖胺、葡糖胺、甘露糖和N - 乙酰神经氨酸。从IgG分子中分离出的糖肽甚至更有效。在培养过程中从DCH - 5细胞释放并通过固定化IgG上的亲和色谱逐渐分离的Fc受体，与聚集的IgG反应并抑制玫瑰花结形成。用单糖洗脱的Fc受体主要影响由IgA和IgE介导的玫瑰花结数量，而用甘氨酸 - HCl缓冲液洗脱的Fc受体优先抑制IgG玫瑰花结。如SDS - PAGE所示，用单糖混合物洗脱的级分的异质性显示出一种有效摩尔质量为50 kg/mol的主要成分。甘氨酸 - HCl洗脱液含有55和38 kg/mol的两种主要成分。IgG - Sepharose 4B结合所有级分，但只有50 kg/mol分子的结合可被单糖抑制。
